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Platform Overview

  • About SeqSMART Platform
  • Workflow Overview (From Variant Input to Report Generation)
  • Access Levels and User Roles
  • System Requirements and Supported Browsers
  • Data Security and Compliance (GDPR, CCPA)

Variant Analysis & ACMG Classification

  • Overview of SeqSMART Variant Classification
  • ACMG/AMP Framework in SeqSMART
  • Customizing or Overriding ACMG Results (for Expert Reviewers)

ACMG Criteria Reference Library

  • Population Data: PM2, BA1, BS1, BS2, PS4
  • Computational Predictive Data: PP3, BP4, BP7
  • Functional Evidence: PS3 and BS3
  • Segregation Evidence: PP1 and BS4
  • Cis/Trans Configuration: PM3 and BP2
  • De Novo and Inheritance Pattern: PS2 and PM6
  • PM1 – Variant Located in a Mutational Hotspot or Critical Functional Domain
  • PS1 and PM5 – Same or Similar Amino Acid Changes at the Same Codon
  • PP2 and BP1 – Evaluating Gene-Specific Variant Tolerance
  • PM4 and BP3 – In-Frame Indels and Repeat Regions in Variant Interpretation
  • PVS1 – Interpreting Loss-of-Function (LoF) Variants in SeqSMART
  • Previous Evidence: PP5, BP6, and BP5
  • PP4 – Phenotype Specificity Supporting Variant Pathogenicity

Gene,Transcript & Technical Information

  • Understanding Genetic Constraints
  • Computational Predictive Data

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